SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Szymanowska-Powalowska,Daria.

Background The production of biofuels from renewable energy sources is one of the most important issues in industrial biotechnology today. The process is known to generate various by-products, for example crude glycerol, which is obtained in the making of biodiesel from rapeseed oil. Crude glycerol may be utilized in many ways, including microbial conversion to 1,3-propanediol (1,3-PD), a raw material for the synthesis of polyesters and polyurethanes. Results The paper presents results of a study on the synthesis of 1,3-propanediol from crude glycerol by a repeated batch method with the use of Clostridium butyricum DSP1. Three cycles of fermentation medium replacement were carried out. The final concentration of 1,3-PD was 62 g/L and the maximum...

Tipo: Journal article

Palavras-chave: Environmental stress; Fermentation; High-performance liquid chromatography.

Ano: 2014

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600011

"A comparison between sugar consumption and ethanol production in wort by immobilized Saccharomyces Cerevisiae, Saccharomyces Ludwigii and Saccharomyces Rouxii on Brewer's Spent Grain"

Mohammadi,Aniseh; Razavi,Seyyed Hadi; Mousavi,Seyyed Mohammad; Rezaei,Karamatollah.

The immobilization of Saccharomyces cerevisiae DSM 70424, Saccharomyces ludwigii DSM 3447 and Saccharomyces rouxii DSM 2531 on brewer's spent grain and then ethanol production and sugar consumption of these immobilized yeasts were investigated. The aim of this study was to investigate the abilities of these three immobilized yeasts for producing alcohol for brewing at two temperatures (7 and 12 °C) using two different sugar levels (one at original level supplied in the brewery and one with 2.5% (w/v), added glucose to the wort). Increasing both parameters resulted in higher alcohol production by all the yeasts studied. At 7 °C and with original wort density the ethanol content at the end of fermentation was 2.7% (v/v) for S. cerevisiae, 1.7% for S....

A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol

Palavras-chave: Brewer's spent grain; Fermentation; Immobilization; Saccharomyces cerevisiae; Saccharomyces ludwigii; Saccharomyces rouxii.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200025

BABT

Anschau,Andréia; Santos,Lucielen Oliveira dos; Alegre,Ranulfo Monte.

This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids...

A new alkalophilic isolate of Bacillus as a producer of cyclodextrin glycosyltransferase using cassava flour

Palavras-chave: Glutathione; Fermentation; Fed Batch Culture; Amino Acids; Response Surface Methodology.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500017

Coelho,Sheila Lorena de Araújo; Magalhães,Valter Cruz; Marbach,Phellippe Arthur Santos; Cazetta,Marcia Luciana.

Abstract Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into non-reducing cyclic sugars, cyclodextrins, which have several industrial applications. This study aimed to establish optimal culture conditions for β-CGTase production by Bacillus sp. SM-02, isolated from soil of cassava industries waste water lake. The optimization was performed by Central Composite Design (CCD) 2, using cassava flour and corn steep liquor as substrates. The maximum production of 1087.9 U mL−1 was obtained with 25.0 g L−1 of cassava flour and 3.5 g L−1 of corn steep after 72 h by submerged fermentation. The enzyme showed optimum activity at pH 5.0 and temperature 55 °C, and maintained thermal stability at 55 °C for 3 h. The enzymatic activity was...

Acetoin: Production of acetoin from glycerin.

Palavras-chave: Microbial enzyme; Fermentation; Agro-industrial substrates.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100120

Infoteca-e

SALUM, T. F. C..

Fermentative process for acetoin production from crude glycerin from biodiesel production using a new bacterial strain.

Tipo: Folders

Palavras-chave: Biocombustível; Acetoin; Fermentation; Biodiesel.

Ano: 2021

URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1132995

Aerobic stability in corn silage (Zea maysL.) ensiled with different microbial additives

Animal Sciences

Bumbieris Junior, Valter Harry; Guimarães, Vinícius André de Pietro; Fortaleza, Ana Paula de Souza; Massaro Junior, Fernando Luiz; Moraes, Gabriella Jorgette de; Meza, Diego Armando Rojas.

This study aimed to assess different microbial additives, regarding the efficiency of aerobic stability in corn silages. The corn hybrid used for silages production was the DKB 310. The treatments consisted of: 1) control treatment without any microbial additive; 2) Treatment with LPPA composed of: Lactobacillus plantarum CCT 0580 3.1 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 3.1 x 1010 CFU g-1; 3) Treatment with Inoculum, composed of Bacillus subtilis CCT 0089 3.0 x 109 CFU g-1, Lactobacillus plantarum CCT 0580 1.2 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 1.5 x 1010 CFU g-1 and 4) Treatment LB, composed only of Lactobacillus buchneri CCT 3746 2.6 x 1010 CFU g-1. The experimental design was completely randomized...

Aerobic stability of sugar cane silages with a novel strain of Lactobacillus sp. isolated from sugar cane

Palavras-chave: 5.04.04.01-6 A aerobiosis; Silage; Fermentation; Nutritional quality..

Ano: 2017

URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/34426

R. Bras. Zootec.

Ávila,Carla Luiza da Silva; Pinto,José Cardoso; Oliveira,Dâmiany Pádua; Schwan,Rosane Freitas.

The objective of this research was to evaluate the effect of the addition of two Lactobacillus strains on the aerobic stability of the sugar cane (Saccharum spp.) silages. The experiment was carried out in a completely randomized design with three replicates in split-plot in time scheme, so that the treatments (silages without inoculant and with novel strain or commercial inoculants) were randomized on plot and in times of aerobic evaluation of the silages (zero, two, four, and six days after the opening of the silos) as subplots. The loss of stability of silages was continuous during the time of aerobic exposure. Inoculants influenced the changes in populations of lactic acid bacteria and yeasts and in the ammoniacal nitrogen, acetic acid and ethanol...

Alcohol production from sweet potato (Ipomoea batatas (L.) Lam.) genotypes in fermentative medium

Palavras-chave: Acetic acid; Fermentation; Lactic acid; Microbiology.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200003

Acta Agron. (Palmira)

Cantos-Lopes,Amanda; Vilela-de Resende,Juliano Tadeu; Machado,José; Perez-Guerra,Edson; Vilela-Resende,Nathalia.

Abstract Ethanol is one of the most promising fuels in Brazil, but its production is centralized in sugarcane cultivation, and is currently in search for other sources alcohol producing biomass to replace sugarcane monoculture. The aim of this study was to study fermentation process variables such as fermentation time (72 and 96 hours), addition of nutrients (with and without addition) and yeasts (dry and fresh organic yeast), employed in ethanol production from two sweet potato (Ipomoea batatas (L.) Lam.) genotypes: UGA 05 and UGA 77. Alcohol evaluation content in °GL was performed in a completely randomized design according to two factorial schemes (22 and 23), and results obtained were analyzed using Design Expert® software version 8. Results of the...

URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122018000200231

Palavras-chave: Biofuel; Ethanol production; Fermentation; Saccharification; Starch; Water content.

Ano: 2018

Alcoholic fermentation by the wild yeasts under thermal, osmotic and ethanol stress

BABT

Silva,Rosimeire Oenning da; Batistote,Margareth; Cereda,Marney Pascoli.

This study aimed to explore the variability in the metabolism of nine wild yeasts isolated from the sugarcane juice from a distillery in the Brazilian State of Mato Grosso. Cell viability under the stress conditions was evaluated. The yeasts were inoculated in the test tubes containing sugarcane juice adjusted from 12 to 21º Brix, ethanol from 6 to 12% in volume and temperature at 30, 35 and 40ºC. The viability was established by the growth in Petri dishes and visually by the CO2 production in the test tubes. None of the evaluated yeasts showed simultaneous resistance to the three stress conditions. The potential of yeast BB.09 could be emphasized due to its ability to ferment up to12% ethanol at 30°C.

Alkali pretreated of wheat straw and its enzymatic hydrolysis

Palavras-chave: Bio prospection; Alcohol distillery; Cellular viability; Fermentation.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000200001

Han,Lirong; Feng,Juntao; Zhang,Shuangxi; Ma,Zhiqing; Wang,Yonghong; Zhang,Xing.

The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose...

Analysis of batch and repeated fedbatch productions of Candida utilis cell mass using mathematical modeling method

Palavras-chave: Cellulase; Cellulose; Fermentation; Hydrolysis; Lignocellulosic; Pretreatment.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100006

ANTIBIOTIC PRODUCTION BY STREPTOMYCES HYGROSCOPICUS D1.5: CULTURAL EFFECT

Lin,Yutian; Liu,Guoli; Lin,Huibin; Gao,Ling; Lin,Jianqiang.

Background: Candida utilis is widely used in bioindustry, and its cell mass needs to be produced in a cost effective way. Process optimization based on the experimental results is the major way to reduce the production cost. However, this process is expensive, time consuming and labor intensive. Mathematical modeling is a useful tool for process analysis and optimization. Furthermore, sufficient information can be obtained with fewer experiments by using the mathematical modeling, and some results can be predicted even without doing experiments. Results: In the present study, we performed the mathematical modeling and simulation for the cell mass production of Candida utilis based on limited batch and repeated fedbatch experiments. The model parameters...

Tipo: Journal article

Palavras-chave: Candida utilis; Fermentation; Genetic algorithm; Mathematical model.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400002

Rev. Microbiol.

Bhattacharyya,Barun K.; Pal,Sushil C.; Sen,Sukanta K..

In an attempt to screen out new potent antibiotic producers from soil, Streptomyces hygroscopicus D1.5 was isolated and was found antagonistic to both bacteria and fungi. It could utilise arginine as nitrogen source and glycerol as carbon source at 0.75 g/l and 11.5 g/l level, respectively for maximum antibiotic yield.

Assessing the nutritional value of agroindustrial co-products and feed through chemical composition, in vitro digestibility, and gas production technique

Palavras-chave: Antibiotics; Fermentation; Streptomyces.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300003

Animal Sciences

Olivo, Paula Martins; Santos, Geraldo Tadeu dos; Ítavo, Luís Carlos Vinhas; Silva Junior, Ranulfo Combuca da; Leal, Eduardo Souza; Prado, Rodolpho Martin do.

Agroindustrial co-products are a viable alternative for use in animal nutrition. Tests were conducted using eight different types of co-products and feed to evaluate the chemical composition, in vitro digestibility of dry matter, crude protein and neutral detergent fiber, and gas production by them. The co-products tested were: coffee hulls; pelleted citrus pulp; grape residue; soybean hulls; cottonseed; cassava foliage; and foods usually supplied to ruminants: corn silage and ground corn concentrate. Data of in vitro digestibility of dry matter, crude protein and neutral detergent fiber were tested by analysis of variance using the least square method; the results of gas production were interpreted by a non-linear regression by the Gauss-Newton method;...

Bioconversion of mixed free fatty acids to poly-3-hydroxyalkanoates by Pseudomonas putida BET001 and modeling of its fermentation in shake flasks

Palavras-chave: Zootecnia; Nutrição de Ruminantes alternative feed; Degradation; Fermentation; Gas production.s.

Ano: 2017

URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/34024

Makhdum Munawar,Khalil Munawar; Simarani,Khanom; Annuar,Mohamad Suffian Mohamad.

Background: The paper reports on the utilization of palm kernel oil (PKO) as a low cost renewable substrate for medium-chain-length poly-3-hydroxyalkanoates (mcl-PHA) production by Pseudomonas putida BET001. Investigation on the effects of selected key variables on growth, mixed free fatty acids consumption and mcl-PHA production by the bacterial culture in the shaken flask system were carried out along with its kinetic modeling. Results: The biomass production, fatty acids consumption and mcl-PHA production were found favorable when the strain was cultured in mineral medium at pH 6-7,28°C, aeration surface-to-volume ratio of 0.4 x 10(6) m-1, 250 rpm agitation rate for 48 h. Mcl-PHA production by this strain showed mixed growth and non-growth associated...

Tipo: Journal article

Palavras-chave: Biopolymer; Fermentation; Medium-chain-length PHA; Palm kernel oil; Pseudomonas putida.

Ano: 2016

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100008

Bioethanol production from coconut husk fiber

Ciência Rural

Cabral,Mirelle Márcio Santos; Abud,Ana Karla de Souza; Silva,Carlos Eduardo de Farias; Almeida,Renata Maria Rosas Garcia.

ABSTRACT: Population growth and the increasing search for healthy foods have led to a major consumption of coconut water and, hence, to an environmental impact caused by the inappropriate disposal of green coconut husks. This lignocellulosic biomass has deserved attention of researchers concerning the seeking of new usages, as, for example, in renewable fuels production technologies. This study examines the potential of green coconut husk fibers as a feedstock for the production of bioethanol. The coconut fibers were pretreated through an alkaline method, hydrolyzed enzymatically and submitted to ethanol fermentation with commercial yeasts of Saccharomyces cerevisiae. Despite the significant loss of cellulose (4.42% in relation to the fiber and 17.9%...

Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance

Palavras-chave: Reuse; Coconut husk; Fermentation; Bioethanol.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001001872

Reis,Vanda Renata; Antonangelo,Ana Teresa Burlamaqui Faraco; Bassi,Ana Paula Guarnieri; Colombi,Débora; Ceccato-Antonini,Sandra Regina.

Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The...

Biomass distribution and physiological capabilities of bacteria in the water column above a sea grass system.

Palavras-chave: Fermentation; Stresses; Yeasts; Microsatellite; Contaminants.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268

ArchiMer

Velimirov, B; Herndl, G; Kavka, G.

The distribution pattern of bacteria in the water column above and within a Posidonia oceanica bed near Ischia (off the Gulf of Naples) was investigated and correlated with DOC and POM data for autumn and winter. Data from samples of 5 stations along a transect from shallow to deep showed rather low bacterial densities (10 super(4).ml super(-1)) for both seasons. The number of attached bacteria was higher in late winter as compared to autumn when the leaves are shed from the stands. Except for occasional peaks DOC ranged between 1.5 and 4.5 mg C/1 while POM concentrations ranged form 4 to 14 mg AFDW.1 super(-1). Hydrolytic capabilities of isolated bacterial colonies showed that more carbohydrate substrates could be fermented in winter as compared to...

Tipo: Text

Palavras-chave: Posidonia oceanica; Microorganisms; Physiology; Seasonal distribution; Algae; Bacteria; Particulate organic matter; Metabolism; Water column; Fermentation; Sea grass.

Ano: 1984

URL: http://archimer.ifremer.fr/doc/1984/acte-954.pdf

Bionanoparticles, a green nanochemistry approach

Cauerhff,Ana; Castro,Guillermo R.

Background: In the past decade, considerable attention has been paid for the development of novel strategies for the synthesis of different kind of nano-objects. Most of the current strategies are usually working by the use physical or chemical principles to develop a myriad of nano-objects with multiple applications. Main fields of nanotechnology applications range from catalysis, micro- and nano-electronics (semiconductors, single electrons transistors), non-linear optic devices, photo-electrochemistry to biomedicine, diagnostics, foods and environment, chemical analysis and others. Results: Two main avenues for nanoparticles synthesis: cell-free extract and cell cultivation have been reported. The state of art of both biotechnological approaches for...

Tipo: Journal article

Palavras-chave: Bacteria; Bionanoparticles; Fermentation; Fungi green chemistry; Plant extracts.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300011

Biotechnological production of citric acid

Max,Belén; Salgado,José Manuel; Rodríguez,Noelia; Cortés,Sandra; Converti,Attilio; Domínguez,José Manuel.

This work provides a review about the biotechnological production of citric acid starting from the physicochemical properties and industrial applications, mainly in the food and pharmaceutical sectors. Several factors affecting citric acid fermentation are discussed, including carbon source, nitrogen and phosphate limitations, pH of culture medium, aeration, trace elements and morphology of the fungus. Special attention is paid to the fundamentals of biochemistry and accumulation of citric acid. Technologies employed at industrial scale such as surface or submerged cultures, mainly employing Aspergillus niger, and processes carried out with Yarrowia lipolytica, as well as the technology for recovering the product are also described. Finally, this review...

Blackberry Vinegar Produced By Successive Acetification Cycles: Production, Characterization And Bioactivity Parameters

Palavras-chave: Citric acid; Fermentation; Review; Aspergillus niger.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400005

BABT

Cunha,Mário Antônio Alves da; Lima,Kely Priscila de; Santos,Vidiany Aparecida Queiroz; Heinz,Otto Lucas; Schmidt,Carla Adriana Pizarro.

ABSTRACT: Blackberry vinegar was produced in successive acetification cycles and content of total phenolics, anthocyanins and antioxidant activity were evaluated along the production. Firstly, blackberry wine was obtained in bench-scale bioreactor, being verified 0.39 g/g ethanol yield, 1.78 g/L.h volumetric productivity and 76% efficiency. After, three successive acetification cycles were conducted efficiently in grapia barrel with average acetic acid production of 51.6 g/L, 72.2 % acetic acid yield and 0.4 g/L.h volumetric productivity. Appreciable contents of polyphenolic compounds, anthocyanins and high antioxidant activity were observed in the raw material, wine and vinegar obtained in each cycle of acetic acid transformation. Acetic acid...